Journal: Clinical Neuropathology

Article Title: Clinical Neuropathology practice guide 5-2015: MGMT methylation pyrosequencing in glioblastoma: unresolved issues and open questions

doi: 10.5414/NP300904

Figure Lengend Snippet: Summary of all publications in which the MGMT promoter methylation was analyzed by pyrosequencing in human glioblastoma tissue. The data include: the number of glioblastoma samples analyzed by pyrosequencing and the fixation method, study type (whether prospective or retrospective and whether a clinical trial with homogeneous therapy or not), analyzed region (as the consecutive CpG numbers within the MGMT CpG island), applied threshold (all values listed if samples were divided into more than 2 groups; if different threshold values were used for the various sites, the range is shown in parentheses).

Article Snippet: The search for papers within the PubMed database was performed using the terms: “ MGMT pyrosequencing” (76 results), “ MGMT methylation glioma” (613 results), “ MGMT methylation glioblastoma” (482 results), last performed on June 10, 2015.

Techniques: Methylation

Journal: Scientific Reports

Article Title: Investigation of MGMT and DAPK1 methylation patterns in diffuse large B-cell lymphoma using allelic MSP-pyrosequencing

doi: 10.1038/srep02789

Figure Lengend Snippet: (A) Overall survival according to DAPK1 methylation. (B) Overall survival according to rs13300553 genotypes. (C) Overall survival according to allelic DAPK1 methylation patterns in individuals heterozygous for rs13300553.

Article Snippet: The biallelically methylated standards were diluted in unmethylated DNA to 10%, 2.5%, 1.25%, and 0.625% methylation and analyzed using the allelic MGMT and DAPK1 MSP-pyrosequencing assays.

Techniques: Methylation

Journal: Genes

Article Title: Prognostic Analysis of the IDH1 G105G (rs11554137) SNP in IDH-Wildtype Glioblastoma

doi: 10.3390/genes13081439

Figure Lengend Snippet: Pathological and molecular characteristics according to IDH1 G105G SNP status.

Article Snippet: Methyl-guanosine methyl transferase (MGMT) promoter methylation status has been analyzed by pyrosequencing, using a ≥9% average methylation level of CpG islands to define the methylated cases, according to Dunn J et al. [ ].

Techniques: Methylation

Journal: Genes

Article Title: Prognostic Analysis of the IDH1 G105G (rs11554137) SNP in IDH-Wildtype Glioblastoma

doi: 10.3390/genes13081439

Figure Lengend Snippet: PFS analysis of clinical/pathological/molecular features by Cox regression.

Article Snippet: Methyl-guanosine methyl transferase (MGMT) promoter methylation status has been analyzed by pyrosequencing, using a ≥9% average methylation level of CpG islands to define the methylated cases, according to Dunn J et al. [ ].

Techniques: Methylation

Journal: Genes

Article Title: Prognostic Analysis of the IDH1 G105G (rs11554137) SNP in IDH-Wildtype Glioblastoma

doi: 10.3390/genes13081439

Figure Lengend Snippet: DSS analysis of clinical/pathological/molecular features by Cox regression.

Article Snippet: Methyl-guanosine methyl transferase (MGMT) promoter methylation status has been analyzed by pyrosequencing, using a ≥9% average methylation level of CpG islands to define the methylated cases, according to Dunn J et al. [ ].

Techniques: Methylation

Journal: Scientific Reports

Article Title: Investigation of MGMT and DAPK1 methylation patterns in diffuse large B-cell lymphoma using allelic MSP-pyrosequencing

doi: 10.1038/srep02789

Figure Lengend Snippet: The standards having both alleles methylated were serially diluted into unmethylated DNA and tested using the allelic MSP-pyrosequencing assays. The experiments were repeated three times for each of the assays. It can be observed that a signal from both alleles above 25% can be detected down to 0.675% methylation. For MGMT the antisense strand was analyzed. (A) The MGMT assay (B) The DAPK assay.

Article Snippet: Standards of known allelic methylation status were tested using the allelic MGMT and DAPK1 MSP-pyrosequencing assays.

Techniques: Methylation

Journal: Scientific Reports

Article Title: Investigation of MGMT and DAPK1 methylation patterns in diffuse large B-cell lymphoma using allelic MSP-pyrosequencing

doi: 10.1038/srep02789

Figure Lengend Snippet: Clinical characteristics of R-CHOP treated patients as a function of MGMT methylation status

Article Snippet: Standards of known allelic methylation status were tested using the allelic MGMT and DAPK1 MSP-pyrosequencing assays.

Techniques: Methylation

Journal: Scientific Reports

Article Title: Investigation of MGMT and DAPK1 methylation patterns in diffuse large B-cell lymphoma using allelic MSP-pyrosequencing

doi: 10.1038/srep02789

Figure Lengend Snippet: (A) Overall survival according to MGMT methylation status. (B) Overall survival according to rs16906252 genotype.

Article Snippet: Standards of known allelic methylation status were tested using the allelic MGMT and DAPK1 MSP-pyrosequencing assays.

Techniques: Methylation